";s:4:"text";s:4765:" All other reagents used in this study were of analytical grade and purchased from Sigma or Merck Chemicals.All cell cultures were incubated at 37°C in a humidified atmosphere with 5% COThe cytotoxicity assay was determined according to the method described by Mosmann [The glucose utilization in HepG2 cells was determined by the method described by van de VenterThe glucose utilization in L6 myoblasts cells was determined according to the methods described by van de VenterLipid accumulation in 3T3-L1 preadipocytes cells was determined according to the method described by OyedemiThe glucose metabolism assay as a reflection of insulin secretion in INS-1 cells using MTT (tetrazolium) colorimetric assay was determined according to the method described by Janjic and Wollheim [The cell proliferation assay was carried out using a method described by Sirenko et al. Our results indicated that the extract demonstrated no significant inhibition on alpha-amylase, glucosidase, lipase, and DPP-IV when compared to the respective positive controls.
It has also been indicated that stimulation of improved glucose metabolism leading to enhancing ATP production in pancreatic Several lines of studies have indicated that the decline in pancreatic beta-cell mass, through either an increase in apoptosis or a decrease in proliferation, is believed to be one of the major contributory factors in the development of type 2 diabetes [Nitric oxide has been reported to contribute to the pathogenesis of diabetes [Presently, there are several antidiabetic drugs used to treat or manage diabetes and the mechanisms of action of these drugs are well known.
In the present study, cytotoxicity effect indicated that less than 50% cell death was recorded for HepG2 cells treated with the extract even at the highest dose of 200 The present study has employed various biochemical and cell-based assays to identify the potential mechanism(s) of probable antidiabetic actions of extract prepared fromThe ability of adipose tissue to accommodate excess lipid can be exceeded in patients suffering from obesity resulting in the abnormal accumulation of lipid in other tissues, such as muscle, liver, and pancreatic islet leading to physiological dysfunction (lipotoxicity) [In pancreatic beta cells, glucose metabolism is very important for glucose stimulated-insulin release.
Idowu Jonas Sagbo, Maryna van de Venter, Trevor Koekemoer, Graeme BradleyIn South Africa, the number of people suffering from diabetes is believed to be rising steadily and the current antidiabetic therapies are frequently reported to have adverse side effects. (C) Diagram of α cell distribution. The aims of this study were to determine the antidiabetic activity of aqueous extract of leaf (LE) and fruit (FE) from P. guajava.
(A‐B) Digital images of whole pancreatic sections stained with H&E (A) or double‐stained with insulin and glucagon (B) were acquired. The data were expressed as the mean ± standard deviation and values were considered significant at p < 0.05.The results obtained for glucose uptake in HepG2 cells in the presence of the plant extract at 25 and 100 The treatments of the extract and insulin in the L6 cell determined by the MTT assay indicated no potential toxicity as shown in Figure The results revealed that the extract displayed a weak significant inhibition against protein glycation at all the tested concentration in a dose-dependent manner.
Box X1314, Alice, South AfricaDepartment of Biochemistry and Microbiology, Nelson Mandela Metropolitan University, P.O. In the in vitro assays, FEM demonstrated a strong antioxidant effect, especially in DPPH scavenging activity and reducing power.